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307 次辣椒素借 SIRT3 抑制鐵死亡維持線粒體穩(wěn)態(tài)，減輕呼吸機(jī)致肺損傷 2025-3-10
Capsaicin mitigates ventilator-induced lung injury by suppressing ferroptosis and maintaining mitochondrial redox homeostasis through SIRT3-dependent mechanismsKeywords: Capsaicin;
350 次腫瘤相關(guān)炎性細(xì)胞因子增強(qiáng)了骨髓間充質(zhì)基質(zhì)細(xì)胞對中性粒細(xì)胞的保護(hù) 2025-3-3
Protection of neutrophils by bone marrow mesenchymal stromal cells is enhanced by tumor-associated inflammatory cytokinesKeywords: G-CSF; inflammation; mesenchymal stromal/stem cel
319 次機(jī)械力調(diào)節(jié)脂肪干細(xì)胞的旁分泌功能促進(jìn)大鼠皮膚擴(kuò)張 2025-3-3
Mechanical force regulates the paracrine functions of ADSCs to assist skin expansion in ratsKeywords: ADSC paracrine; Angiogenesis; Collagen secretion; Mechanical force; Skin expan
387 次 GDF15 調(diào)節(jié)唑來膦酸誘導(dǎo)的牙周膜成纖維細(xì)胞的高炎機(jī)械反應(yīng) 2025-2-24
GDF15 Modulates the Zoledronic-Acid-Induced Hyperinflammatory Mechanoresponse of Periodontal Ligament FibroblastsKeywords: DNA damage response; GDF15; inflammatory mechanoresponse;
373 次 EVA1A通過調(diào)控自噬在擾動(dòng)流下促進(jìn)內(nèi)皮細(xì)胞凋亡和炎癥激活 2025-2-24
EVA1A (Eva-1 Homolog A) Promotes Endothelial Apoptosis and Inflammatory Activation Under Disturbed Flow Via Regulation of AutophagyKeywords: apoptosis; atherosclerosis; autophagy;
479 次國產(chǎn)蛋白純化儀做離子交換層析實(shí)驗(yàn)指南 2025-2-18
離子交換層析實(shí)驗(yàn)指南離子交換層析（Ion Exchange Chromatography, IEX）是一種基于蛋白等電點(diǎn)（pI）和緩沖液 pH 之間的電荷差異，利用離子交換介質(zhì)對帶正電（陽離子交換）或負(fù)電（陰離子交換）
570 次提問DeepSeek：國產(chǎn)生物儀器發(fā)展策略分析 2025-2-18
生物儀器的國產(chǎn)替代是中國科技自立自強(qiáng)的重要戰(zhàn)略方向，結(jié)合當(dāng)前政策支持、技術(shù)突破與市場需求，其未來發(fā)展路徑和趨勢可總結(jié)如下：一、國產(chǎn)替代的核心驅(qū)動(dòng)力1. 政策支持與技術(shù)攻關(guān)國家通過多項(xiàng)政
412 次 EPAS1 通過內(nèi)皮脂肪酸攝取減輕血流紊亂區(qū)域動(dòng)脈粥樣硬化的發(fā)生 2025-2-17
EPAS1 Attenuates Atherosclerosis Initiation at Disturbed Flow Sites Through Endothelial Fatty Acid UptakeKeywords: atherosclerosis; diet, high-fat; endothelial cells; obesity; plaq
483 次 NADPH 氧化酶4在缺氧條件下介導(dǎo)內(nèi)皮功能 2025-2-17
NADPH Oxidase 4: Crucial for Endothelial Function under Hypoxia—Complementing ProstacyclinKeywords: NADPH oxidase 4; PTGIS; endothelial function; human endothelial cells; hyp
571 次 EKF 葡萄糖乳酸分析儀的參數(shù)和應(yīng)用 2025-2-10
本文了解EKF葡萄糖乳酸分析儀的詳細(xì)參數(shù)，包括測量范圍、準(zhǔn)確性、樣品用量等，并探索其在體育科學(xué)、食品工業(yè)等領(lǐng)域的廣泛應(yīng)用。一、EKF 葡萄糖乳酸分析儀：技術(shù)參數(shù)與廣泛應(yīng)用EKF 葡萄糖乳酸分析
432 次人臍帶間充質(zhì)干細(xì)胞促進(jìn)小膠質(zhì)細(xì)胞中的細(xì)胞外基質(zhì)重塑 2025-2-10
Human Umbilical Cord-Mesenchymal Stem Cells Promote Extracellular Matrix Remodeling in MicrogliaKeywords: extracellular matrix; human umbilical cord mesenchymal stem cells; microgl
441 次 Piezo1 通道通過介導(dǎo)機(jī)械應(yīng)力誘導(dǎo)的鐵內(nèi)流來加劇髓核細(xì)胞的鐵死亡 2025-2-10
Piezo1 channel exaggerates ferroptosis of nucleus pulposus cells by mediating mechanical stress-induced iron influxSubject terms: Homeostasis, Pathogenesis機(jī)械應(yīng)力可以有效地調(diào)節(jié)細(xì)胞
494 次使用G418篩選HEK-293T細(xì)胞的詳細(xì)步驟 2025-2-8
使用G418篩選HEK-293T細(xì)胞的詳細(xì)步驟1. 細(xì)胞培養(yǎng)：復(fù)蘇HEK-293T細(xì)胞，用合適的培養(yǎng)基（如DMEM高糖培養(yǎng)基，含 10%胎牛血清等）在37℃、5% CO₂培養(yǎng)箱中培養(yǎng)，待細(xì)胞匯合度達(dá)80%-90% 時(shí)進(jìn)行傳
498 次蛋白純化系統(tǒng)如何去做親和實(shí)驗(yàn) 2025-2-8
親和蛋白純化是一種基于蛋白質(zhì)與特定配體之間的特異性結(jié)合的純化技術(shù)，廣泛用于分離具有生物學(xué)功能的目標(biāo)蛋白。以下是具體實(shí)驗(yàn)步驟：以下所用的儀器為輝因科技的蛋白純化系統(tǒng)Hass25，具體配置為
509 次 HEK293培養(yǎng)基適合于不同亞型HEK293細(xì)胞的高密度懸浮培養(yǎng) 2025-2-7
上�；菡\生物提供的HEK293 瞬轉(zhuǎn)表達(dá)基礎(chǔ)培養(yǎng)基（Basal Medium）與補(bǔ)料培養(yǎng)基（Feed Medium）是完全化學(xué)成分限定（Chemically Defined），均不含血清、水解物及任何動(dòng)物來源的成分，適合于不同亞
475 次硫酸化K5多糖具有抑制腫瘤細(xì)胞增殖和轉(zhuǎn)移的潛力 2025-1-7
硫酸化K5多糖是一種經(jīng)過硫酸化修飾的K5多糖。K5多糖是一種天然存在的多糖，主要來源于大腸桿菌（Escherichia coli）的K5莢膜多糖。K5多糖的結(jié)構(gòu)與肝素（heparin）的前體物質(zhì)非常相似，因此具有潛
530 次 B細(xì)胞前體急性淋巴細(xì)胞白血病在骨髓間充質(zhì)基質(zhì)中引發(fā)干擾素α/β反應(yīng) 2024-12-30
B-cell precursor acute lymphoblastic leukemia elicits an interferon-α/β response in bone marrow-derived mesenchymal stromaKWS：BCP-ALL；interferon-α/β；bone m
545 次機(jī)械力經(jīng)靶向 SMAD1促巨噬細(xì)胞外泌體 UCHL3促進(jìn)骨髓間充質(zhì)干細(xì)胞成骨 2024-12-30
Mechanical force induces macrophage-derived exosomal UCHL3 promoting bone marrow mesenchymal stem cell osteogenesis by targeting SMAD1Keywords: Exosomes; Macrophages; Mechanical fo
388 次人牙齦成纖維細(xì)胞的成骨分化可抑制破骨細(xì)胞形成 2024-12-23
Osteogenic Differentiation of Human Gingival Fibroblasts Inhibits Osteoclast FormationKeywords: gingival fibroblasts; osteoblasts; osteoclastogenesis; osteoclasts; osteogenesis.牙齦
380 次循環(huán)牽張應(yīng)力通過整合素αVβ3-肌動(dòng)蛋白絲軸調(diào)控MSCs早期成骨分化 2024-12-23
Regulation of the integrin αVβ3- actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stressKeywords: Integrin &