全自動Digital WB系統(tǒng)與Merck十年合作優(yōu)化發(fā)展史

自2011年，ProteinSimple上市第一代全自動Digital Western Blot系統(tǒng)Simon以來，十年間，我們不斷傾聽客戶的聲音，不斷創(chuàng)新優(yōu)化，推出Sally Sue、Wes、Peggy Sue、NanoPro1000、Jess和Abby系統(tǒng)。
 

在生物制品蓬勃發(fā)展的十年中，無論是“百抗”、雙抗、ADC、PROTAC、AbTACs（雙特異性抗體+PROTACs）、疫苗（埃博拉疫苗、肺炎疫苗、人類巨細(xì)胞病毒疫苗、四價艱難俊菌疫苗）、細(xì)胞和基因治療領(lǐng)域，ProteinSimple全自動Digital Western Blot都躬身入局。
 

Merck作為全球制藥企業(yè)，十年間，ProteinSimple與Merck深度合作，助力藥物研發(fā)（藥效動力學(xué)、靶點驗證等等）、疫苗研發(fā)和生產(chǎn)。包括，全球埃博拉疫苗ERVEBO®和15價肺炎鏈球菌疫苗上市。僅在Merck美國賓夕法尼亞州West Point，一個Site，就有26臺全自動Digital Western Blot系統(tǒng)運行。全球范圍內(nèi)Merck多個Site和部門，也都遍地開花。
 

ProteinSimple全自動Digital Western Blot系統(tǒng)，以其高度自動化、高質(zhì)量數(shù)據(jù)全面助力生物制品行業(yè)上游和下游相關(guān)工作。
 

在此，分享Merck公司利用全自動Digital WB系統(tǒng)，十年間，發(fā)表過的18篇文章。

2021

Establishing and Validating Cellular Functional Target Engagement Assay for Selective IRAK4 Inhibitor Discovery

全自動Digital Western Blot檢測系統(tǒng)：Peggy Sue

藥物研發(fā)：藥效動力學(xué)

Site：Department of Quantitative Bioscience&Department of Discovery Chemistry, Merck & Co., Inc., Boston, MA, USA

Development of a novel capillary electrophoresis-based approach for detection of anti-drug antibody responses

全自動Digital Western Blot檢測系統(tǒng)：Peggy Sue

藥物發(fā)現(xiàn)：臨床前ADA（ Anti-Drug Antibodies） 分析鑒定

Site：PPDM&Biologics, Merck & Co., Inc., 213 E Grand Ave, South San Francisco, CA 94080, USA 

Characterization of gH/gL/pUL128-131 pentameric complex, gH/gL/gO trimeric complex, gB and gM/gN glycoproteins in a human cytomegalovirus using automated capillary western blots

全自動Digital Western Blot檢測系統(tǒng)：Wes

人類巨細(xì)胞病毒疫苗：定量人巨細(xì)胞病毒疫苗多種抗原水平

Site: Vaccine Analytical Research Development and Vaccine Process Development Merck & Co., Inc., Kenilworth, NJ, USA
2020

Characterization of rVSVΔG-ZEBOV-GP glycoproteins using automated capillary western blotting

全自動Digital Western Blot檢測系統(tǒng)：Wes

埃博拉疫苗：全球上市預(yù)防埃博拉病毒病的商品化疫苗ERVEBO®
Site: Vaccine Analytical Research Development and Vaccine Process Development and Commercialization, Merck & Co., Inc., Kenilworth, NJ, USA

A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice

全自動Digital Western Blot檢測系統(tǒng)：Peggy Sue

藥物靶點驗證

Site：Translational Innovation Platform Oncology, Merck KGaA, Darmstadt, Germany等

A capillary electrophoresis based approach for the identification of anti-drug antibodies against camelid VHH biologics (Nanobodies®)

全自動Digital Western Blot檢測系統(tǒng)：Wes

藥物發(fā)現(xiàn)：針對Nanobody的ADA鑒定

Site:  PPDM ADME Biologics, PPDM Bioanalytics, Immunology and IMRs, MRL, Merck & Co., Inc., 213 E. Grand Avenue, South San Francisco, CA 94080, USA

Development of an ADP-ribosylation assay for residual toxicity in C.difficile binary toxin CDTa using automated capillary western blot

全自動Digital Western Blot檢測系統(tǒng)：Peggy Sue

四價艱難俊菌疫苗：ADP 核糖基化活性

Site: Vaccine Research Analytical Development, Merck & Co., Inc., Kenilworth, NJ, USA
2019

Efficacy and Pharmacodynamic Modeling of the BTK Inhibitor Evobrutinib in Autoimmune Disease Models

全自動Digital Western Blot檢測系統(tǒng)：Wes

藥物發(fā)現(xiàn)：BTK抑制劑Evobrutinib

Site: Translational Innovation Platform Immunology, Merck KGaA, Darmstadt 64293,

Germany; Immunology, Merck Serono, 1201 Geneva, Switzerland等
2017

Quantitation of CRM197 using imaged capillary isoelectric focusing with fluorescence detection and capillary Western

全柱成像毛細(xì)管等電聚焦電泳系統(tǒng)：Maurice

全自動Digital Western Blot檢測系統(tǒng)：Wes

疫苗生產(chǎn)雜質(zhì)鑒定：白喉毒素

Site：Biologics and Vaccines 8 Merck Sharp & Dohme, West Point, PA 19486, USA

An integrin antagonist (MK‐0429) decreases proteinuria and renal fibrosis in the ZSF1 rat diabetic nephropathy model

全自動Digital Western Blot檢測系統(tǒng)：Sally Sue

藥物研發(fā)：腎病藥物

Site：Department of Pharmacology, Department of Cardiometabolic Diseases&Department of Medicinal Chemistry, Merck & Co., Inc., 2000 Galloping Hill Road, Kenilworth, New Jersey 07033

Neutralization of Diverse Human Cytomegalovirus Strains Conferred by Antibodies Targets Viral gH/gL/pUL128-131 Pentameric Complex

全自動Digital Western Blot檢測系統(tǒng)：Simon

人類巨細(xì)胞病毒疫苗：抗體檢測

Site：Merck Research Laboratories, Merck and Co., Inc., Kenilworth, New Jersey, USA等

Ability of Bruton’s Tyrosine Kinase Inhibitors to Sequester Y551 and Prevent Phosphorylation Determines Potency for Inhibition of Fc Receptor but not B-Cell Receptor Signaling

全自動Digital Western Blot檢測系統(tǒng)：Wes

免疫學(xué)：Btk（布魯頓酪氨酸激酶）磷酸化檢測

Site：TIP Immunology, Merck, Darmstadt, Germany等
2016

Applications of an Automated and Quantitative CE-Based Size and Charge Western Blot for Therapeutic Proteins and Vaccines

全自動Digital Western Blot檢測系統(tǒng)：Sally, Sally Sue, Peggy, Peggy Sue, Wes

四價艱難梭菌疫苗: 篩選細(xì)胞克隆株、免疫學(xué)鑒別和穩(wěn)定性研究

Site: NA

2015

Automated capillary Western dot blot method for the identity 4 of a 15-valent pneumococcal conjugate vaccine

全自動Digital Western Blot檢測系統(tǒng)：Peggy Sue

15價肺炎鏈球菌疫苗免疫學(xué)鑒別

Site: Vaccine Analytical Development, Merck Research Laboratories, West Point, PA 19486, USA

Soluble Human Cytomegalovirus gH/gL/pUL128-131 Pentameric Complex, but not gH/gL, Inhibits Viral Entry to Epithelial Cells and Presents Dominant Native Neutralizing Epitopes

全自動Digital Western Blot檢測系統(tǒng)：NanoPro

人類巨細(xì)胞病毒疫苗：gH/gL 和gH/gL/pUL128–131五聚體蛋白檢測

Site: Vaccine Bioprocess Research & Development, Merck Research Laboratories, West Point, PA 19486, USA
2014

Residual bovine serum albumin (BSA) quantitation in vaccines using automated Capillary Western technology

型號：NA

疫苗牛血清白蛋白（BSA）雜質(zhì)檢測

Site：Vaccine Analytical Development, Merck Research Laboratories, West Point, PA 19486, USA

Effects of anacetrapibon plasma lipids, apolipoproteins and PCSK9 in healthy,lean rhesus macaques

全自動Digital Western Blot檢測系統(tǒng)：Sally Sue

心血管藥理學(xué)：低密度脂蛋白受體蛋白水平檢測

Site: Merck Research Laboratories, Merck & Co., Inc., 2000 Galloping Hill Road, Kenilworth, NJ 07033, USA
2012

Qualitative and quantitative evaluation of SimonTM, a new CE-based automated Western blot system as applied to vaccine development

全自動Digital Western Blot檢測系統(tǒng)：Simon

候選疫苗定量檢測：CV ＜ 10%

Site: Vaccine Analytical Development, Merck Research Laboratories, West Point, PA, USA