高通量葉綠素?zé)晒獬上衿脚_(tái)在微生物與表型組學(xué)領(lǐng)域應(yīng)用

高通量葉綠素?zé)晒獬上衿脚_(tái)在微生物與表型組學(xué)領(lǐng)域應(yīng)用

WIWAM XY植物表型成像系統(tǒng)（定制版）

含GFP蛋白的植物RGB圖像； Pathoviewer多光譜相機(jī)圖像.

含GFP的小麥RGB 圖像； Pathoviewer多光譜相機(jī)圖像.

WIWAM植物表型成像分析系統(tǒng)由Ghent大學(xué)的VIB研究所、世界先進(jìn)的自動(dòng)化工程公司SMO聯(lián)合歐洲先進(jìn)科學(xué)家合作研制生產(chǎn)的新型植物表型組學(xué)研究平臺(tái)，可以全自動(dòng)、高通量、高容量測(cè)量分析擬南芥、水稻、玉米、大豆等各種植物的三維表型形狀及生理狀態(tài)及其與環(huán)境條件關(guān)系。

WIWAM植物表型成像分析系統(tǒng)可集成各種成像模塊，如自動(dòng)化控制系統(tǒng)、熒光成像測(cè)量分析（包括葉綠素、紅熒光）、植物紅外熱成像分析、植物近紅外成像分析、植物多光譜分析、植物高光譜分析、自動(dòng)條碼識(shí)別管理、RGB真彩3D成像、激光3D多光譜成像、計(jì)算機(jī)斷層掃描技術(shù)、自動(dòng)稱重與澆灌系統(tǒng)等多項(xiàng)先進(jìn)技術(shù)，實(shí)現(xiàn)生理生態(tài)與形態(tài)結(jié)構(gòu)成像分析，用于高通量植物表型成像分析測(cè)量、植物脅迫響應(yīng)成像分析測(cè)量、植物生長(zhǎng)分析測(cè)量、生態(tài)毒理學(xué)研究、性狀識(shí)別及植物生理生態(tài)分析研究等。

At the scene of the crime: New insights into the role of weakly pathogenic members of the fusarium head blight disease complex
Jiang Tan ，Maarten Ameye ，Sofie Landschoot ，Noémie De Zutter ，Sarah De Saeger Marthe De Boevre，Mohamed F. Abdallah ，Theo Van der Lee ，Cees Waalwijk ，Kris Audenaert

Abstract
Plant diseases are often caused by a consortium of pathogens competing with one another to gain a foothold in the infection niche. Nevertheless, studies are often limited to a single pathogen on its host. In Europe, fusarium head blight (FHB) of wheat is caused by multiple Fusarium species, including Fusarium graminearum and F. poae. Here, we combined a time series of (co)inoculations, monitored by multispectral imaging, transcriptional, and mycotoxin analyses, to study the temporal interaction between both species and wheat. Our results showed coinoculation of F. graminearum and F. poae inhibited symptom development but did not alter mycotoxin accumulation compared to a single inoculation with F. graminearum. In contrast, preinoculation of F. poae reduced both FHB symptoms and mycotoxin levels compared to a single F. graminearum infection. Interestingly, F. poae exhibited increased growth in dual infections, demonstrating that this weak pathogen takes advantage of its co‐occurrence with F. graminearum. Quantitative reverse transcription PCR revealed that F. poae induces LOX and ICS gene expression in wheat. We hypothesize that the early induction of salicylic and jasmonic acid‐related defences by F. poae hampers a subsequent F. graminearum infection. This study is the first to report on the defence mechanisms of the plant involved in a tripartite interaction between two species of a disease complex and their host.