歐洲科學(xué)家利用WIWAM XY植物表型成像系統(tǒng)發(fā)表文章

歐洲科學(xué)家利用WIWAM XY植物表型成像系統(tǒng)發(fā)表文章 

來自歐洲的科學(xué)家，利用SMO公司構(gòu)建的WIWAM XY植物表型成像系統(tǒng)，發(fā)表了題為Respiratory CO2 Combined With a Blend of Volatiles Emitted by Endophytic Serendipita Strains Strongly Stimulate Growth of Arabidopsis Implicating Auxin and Cytokinin Signaling的文章。WIWAM XY作為一款通用的表型成像系統(tǒng)，已經(jīng)廣泛應(yīng)用于各種小植物表型研究并發(fā)表了系列文章。文章就該系統(tǒng)有以下介紹：
 

多光譜植物表型
實驗中利用該系統(tǒng)進(jìn)行了擬南芥中的花青素和葉綠素含量測量(指數(shù)AriIdx和ChlIdx)并測量了與PSII最大量子效率相關(guān)的葉綠素?zé)晒鈪��?shù)。所有數(shù)據(jù)都由可控環(huán)境中的自動植物表型成像平臺-WIWAM XY 系統(tǒng) (SMO, Eeklo, Belgium) 生成，該系統(tǒng)配備了6-Mp 16-bit 3CCD 頂視相機(jī)，相機(jī)安裝在直角坐標(biāo)機(jī)器人上，用于高通量和高分辨率熒光和多光譜成像。 

北京博普特科技有限公司是比利時SMO公司系列植物表型成像系統(tǒng)中國區(qū)總代理，全面負(fù)責(zé)其系列產(chǎn)品在中國市場的推廣、銷售和售后服務(wù)。

 


Respiratory CO2 Combined With a Blend of Volatiles Emitted by Endophytic Serendipita Strains Strongly Stimulate Growth of Arabidopsis Implicating Auxin and Cytokinin Signaling

Abstract and Figures 
Rhizospheric microorganisms can alter plant physiology and morphology in many different ways including through the emission of volatile organic compounds (VOCs). Here we demonstrate that VOCs from beneficial root endophytic Serendipita spp. are able to improve the performance of in vitro grown Arabidopsis seedlings, with an up to 9.3-fold increase in plant biomass. Additional changes in VOC-exposed plants comprised petiole elongation, epidermal cell and leaf area expansion, extension of the lateral root system, enhanced maximum quantum efficiency of photosystem II (Fv/Fm), and accumulation of high levels of anthocyanin. Notwithstanding that the magnitude of the effects was highly dependent on the test system and cultivation medium, the volatile blends of each of the examined strains, including the references S. indica and S. williamsii, exhibited comparable plant growth-promoting activities. By combining different approaches, we provide strong evidence that not only fungal respiratory CO2 accumulating in the headspace, but also other volatile compounds contribute to the observed plant responses. Volatile profiling identified methyl benzoate as the most abundant fungal VOC, released especially by Serendipita cultures that elicit plant growth promotion. However, under our experimental conditions, application of methyl benzoate as a sole volatile did not affect plant performance, suggesting that other compounds are involved or that the mixture of VOCs, rather than single molecules, accounts for the strong plant responses. Using Arabidopsis mutant and reporter lines in some of the major plant hormone signal transduction pathways further revealed the involvement of auxin and cytokinin signaling in Serendipita VOC-induced plant growth modulation. Although we are still far from translating the current knowledge into the implementation of Serendipita VOCs as biofertilizers and phytostimulants, volatile production is a novel mechanism by which sebacinoid fungi can trigger and control biological processes in plants, which might offer opportunities to address agricultural and environmental problems in the future.