硬山核桃幼樹葉片黃酮醇濃度對養(yǎng)分施肥的響應(yīng)

瀏覽次數(shù)：928　發(fā)布日期：2022-5-10　來源：本站　僅供參考，謝絕轉(zhuǎn)載，否則責(zé)任自負(fù)

熱點(diǎn)
以白僵菌為材料，對生長分化平衡假說（GDBH）進(jìn)行了檢驗(yàn)。
對黃酮醇，觀察到GDBH預(yù)測的非線性“駝峰形”反應(yīng)。
在低碳和黃酮醇的高可用性分配中。

摘要
生長分化平衡假說（GDBH）假設(shè)，類黃酮等植物次生代謝物（PSMs）與氮（N）有效性之間存在非線性“駝峰”反應(yīng)。當(dāng)?shù)挠行灾辽龠_(dá)到五個水平時，這種反應(yīng)最容易被檢測到，但很少有直接或間接測試GDBH的研究使用了三個或更多水平。50棵白樺（marula）幼樹（平均基徑 = 35.5  mm，掃描電鏡 = 0.52; 平均高度 = 80.9 cm，掃描電鏡 = 1.02），隨機(jī)分配到果園中的五種肥料施用率。在生長季節(jié)，每兩個月向每棵樹苗施用含有緩釋硝酸鹽的肥料[N:P:K（3:1:5）（26）]，作為追肥，其施用量相當(dāng)于0、20、40、80和120 G N M−2.在生長季節(jié)，每兩個月使用Dualex®系列4儀器間接測量一次每棵樹苗30–35片最近成熟葉片的平均黃酮含量。觀察到GDBH預(yù)測的非線性“駝峰形”響應(yīng)，表明在高和低養(yǎng)分有效性條件下，碳分配給PSM的范圍比在中等養(yǎng)分有效性條件下更為有限。二次響應(yīng)的成功檢測可能是通過僅測量黃酮醇，這是S.birrea中的主要PSM，以及在肥力極低的土壤上進(jìn)行試驗(yàn)來實(shí)現(xiàn)的。

Foliar flavonol concentration in Sclerocarya birrea saplings responds to nutrient fertilisation according to growth-differentiation balance hypothesis

Highlights
Growth-differentiation balance hypothesis (GDBH) was tested on Sclerocarya birrea.
Nonlinear ‘hump-shaped’ response predicted by GDBH was observed for flavonols.
Allocation of carbon to flavonols limited at low and high nutrient availability.

Abstract
The growth-differentiation balance hypothesis (GDBH) postulates a nonlinear, ‘hump-shaped’ response of plant secondary metabolites (PSMs), such as flavonoids, in relation to nitrogen (N) availability. The response is most easily detected when there are at least five levels of N availability, but very few studies that directly or indirectly tested the GDBH have used three or more levels. Fifty Sclerocarya birrea (marula) saplings (mean basal diameter = 35.5 mm, SEM = 0.52; mean height = 80.9 cm, SEM = 1.02), were randomly assigned to five fertiliser application rates in an orchard. Fertilizer [N:P:K (3:1:5) (26)] containing slow release nitrate was applied to each sapling as a top dressing every two months during the growing season at rates equivalent to 0, 20, 40, 80 and 120 g N m−2. Average flavonoid content of 30–35 recently matured leaves per sapling was indirectly measured every two months during the growing season using the Dualex® Series 4 instrument. The nonlinear, ‘hump-shaped’ response predicted by GDBH was observed, indicating that allocation of carbon to PSMs was more limited at both high and low nutrient availability than at intermediate nutrient availability. Successful detection of the quadratic response was probably facilitated by only measuring flavonols, which are the main PSMs in S. birrea, and by conducting the experiment on soils of very low fertility.

Dualex是一款源自于法國國家科學(xué)院 (CNRS)及巴黎第十一大學(xué)技術(shù)，由奧地利PESSL公司生產(chǎn)（原法國Force-A公司）開發(fā)的新型多功能葉片測量儀。它可同時準(zhǔn)確測量葉片的葉綠素含量、葉片表層的類黃酮和花青素含量，適用于植物生理學(xué)和農(nóng)學(xué)（如水稻葉綠素濃度，玉米氮素狀況，葡萄藤等）相關(guān)研究。其測量對象可以是單子葉植物，雙子葉植物或多年生植物。這款設(shè)備簡單易用，可進(jìn)a行實(shí)時和非破壞性測量。由于不需要校準(zhǔn)標(biāo)定和事先的樣品制備，測量工作可在實(shí)驗(yàn)室或現(xiàn)場完成。此外，該設(shè)備在各種溫度的和環(huán)境光照條件下均可正常使用。系統(tǒng)采用專利設(shè)計(jì)，光學(xué)傳感器可進(jìn)行簡單、快速、無損測量葉片中的葉綠素、多酚以及花青素。

Dualex便攜式智能氣象-葉綠素-花青素-黃酮醇測量儀

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