高分辨磁式質譜儀在環(huán)境領域的應用報告題錄及案例（下）

應用案例三：多環(huán)芳烴

儀器方法：
Injector: Split/splitless,            1 min, 320℃ , 1.5 μL
injection volume with Merlin seal
Carrier gas:                          He, 0.6 mL/min, const. flow
Column:                               TRACE ™ -50MS, 10 m x 0.1 mm x 0.1 μm
Oven Temp. Program:                   140 ℃ , 1 min
                                      10 ℃ /min to 240℃
                                      5 ℃ /min to 270℃
                                      30 ℃ /min to 280℃
                                      4 ℃ /min to 290℃
                                      30 ℃ /min to 315℃
                                      3 ℃ /min to 330℃
Transfer line                         300℃
ion source                            280℃
Ionization:                           EI, 45 eV pos.
Scan Mode:                            Multiple ion detection mode (MID)
Resolution:                           8,000, 10% valley definition
Cycle Time:                           0.8 s/scan

參考文獻
[Application Note: 30126] Fast GC/HRMS Quantification of 16 EC Priority PAH ComponentsKarl Speer, Technical University Dresden, Institute of Food Chemistry, Dresden, Germany, Katja Ziegenhals, Wolfgang Jira, Federal Research Centre for Nutrition and Food (BfEL), Kulmbach, Germany,
Hans-Joachim Huebschmann, Thermo Fisher Scientific, Bremen, Germany
Contents of polycyclic aromatic hydrocarbons (PAH) and phenolic substances in Frankfurter-type sausages depending on smoking conditions using glow smoke
Margarete Pöhlmann , Alexander Hitzel , Fredi Schwägele , Karl Speer , Wolfgang Jira
Polycyclic aromatic hydrocarbons (PAH) and phenolic substances in meat products smoked with different types of wood and smoking spices Alexander Hitzel , Margarete Pöhlmann , Fredi Schwägele , Karl Speer , Wolfgang Jira

SCCP C 10 H 17 Cl 5 –“CP3”標準物質色譜圖

Sample Preparation

樣品準備和標準物質由 Environment Canada, Burlington ON, Canada 提供。
1. 標準物質 SCCP C 10 H 17 Cl 5 – “CP3”
2. 混合物 SCCPs C10-13 55.5% Cl 環(huán)己烷
3. 包含 C13 內標的基質提取樣品

Gas Chromatography

GC Column：Trace Gold TR-5MS
30m x 0.25mm, 0.1 µm film
GC Liner：Single taper splitless
Injector temp： 220 ℃
Splitless time：3 minutes
Transfer line：220℃
Column Flow：1 mLmin -1 , constant flow

Mass Spectrometry

Electron capture negative chemical ionisation
was used to analyse SCCPs.
EC NCI Buffer Gas：Isobutane and ammonia
Source Pressure：6x10-4 mBar
Electron engery：90 eV
Emission current：0.3 mA
Ion source temp：80-95 ℃
Scan Multiple Ion Detection (MID)

參考文獻
Analysis of Short Chain Chlorinated Paraffins (SCCPs) using High Resolution Mass Spectrometry and Negative Chemical Ionization (NCI GC-HRMS)
Frank Theobald1, Heinz Mehlmann2, Dirk Krumwiede2 & Kyle D’Silva2 1. Environmental Consulting, Cologne, Germany 2. POPs Center of Excellence, Thermo Fisher Scientific,Bremen, Germany

應用案例五：荷爾蒙 類固醇及農(nóng)藥

●  EPA Method 1698: Steroids and Hormones in Water, Soil, Sediment,and Biosolids by HRGC/HRMS
高分辨氣相色譜與高分辨磁質譜聯(lián)用分析水體、土壤、沉積物及生物體中的類固醇和荷爾蒙
●  EPA Method 1699: Pesticides in Water,Soil, Sediment, Biosolids, and Tissue by HRGC/HRMS
高分辨氣相色譜與高分辨磁質譜聯(lián)用分析水體、土壤、沉積物及生物體中的農(nóng)藥

參考文獻
●  EPA Method 1698: Steroids and Hormones in Water, Soil, Sediment,and Biosolids by HRGC/HRMS
●  EPA Method 1699: Pesticides in Water,Soil, Sediment, Biosolids, and Tissue by HRGC/HRMS