ChIP-seq測序

ChIP-seq（Chromatin immunoprecipitation followed by sequencing）使用抗體富集的方法在基因組范圍內(nèi)檢測多種蛋白（例如轉(zhuǎn)錄因子CTCF）和其他染色質(zhì)相關(guān)蛋白（例如H3K4me3）與DNA的相互作用。ChIP-seq用于研究表觀基因組如何促進細胞生長，癌癥和其他疾病發(fā)生，已經(jīng)成為確定不同組織、細胞的組蛋白修飾譜的金標準。

IP后DNA：≥10 ng
細胞：5x10⁸
組織：1 g

經(jīng)典案例

1. Collins JM, Huo Z, Wang D. ESR1 ChIP-Seq Identifies Distinct Ligand-Free ESR1 Genomic Binding Sites in Human Hepatocytes and Liver Tissue. Int J Mol Sci. 2021 Feb 2;22(3):1461
2. Namani A, Liu K, Wang S, Zhou X, Liao Y, Wang H, Wang XJ, Tang X. Genome-wide global identification of NRF2 binding sites in A549 non-small cell lung cancer cells by ChIP-Seq reveals NRF2 regulation of genes involved in focal adhesion pathways. Aging (Albany NY). 2019 Dec 28;11(24):12600-12623
3. Karolak JA, Gambin T, Szafranski P, Stankiewicz P. Potential interactions between the TBX4-FGF10 and SHH-FOXF1 signaling during human lung development revealed using ChIP-seq. Respir Res. 2021 Jan 21;22(1):26